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Updated: Sep 27, 2026
Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials
Published on: August 13, 2019
[Experimental study on rabbit periosteal osteoblasts and renal vascular endothelial cells indirect co-culture in
Wei-nan Zhu1, Zhi-ming Yang, Xiu-qun Li
1Key Laboratory of Transplant Engineering and Immunology, Ministry of Public Health: Institute of Reparative and Reconstructive Surgery, Lab of Tissue Engineering, West China Hospital, Sichuan University, Chengdu, Sichuan, P. R. China 610041.
Objective:
To determine an optimal co-culture ratio of the rabbit periosteal osteoblasts (RPOB) and rabbit renal vascular endothelial cells(RRVEC) without direct contact for future study of bone tissue engineering.
Methods:
RPOB and RRVEC in the ratios of 1:0(control group), 2:1(group 1), 1:1(group 2) and 1:2(group 3) were co-cultured by six well plates and cell inserts. Four days later, the proliferation of RPOB and RRVEC were examined through cell count. Differentiated cell function was assessed by alkaline phosphatase (ALP) activity assay and 3H proline incorporation assay.
Results:
When RPOB and RRVEC were indirectly co-cultured, the proliferation of RPOB and 3H proline incorporation was higher in group 1 than in the other experimental groups and control group (P < 0.05). ALP activity of RPOB was higher in group 1 than in control group and group 3 (P < 0.05), but there was no significant difference between group 1 and group 2 (P > 0.05).
Conclusion:
These results suggest that RPOB and RRVEC co-cultured in a ratio of 2:1 is optimal for future study of bone tissue engineering.
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