Related Experiment Videos
[Autocrine regulation for transforming growth factor-beta 1 by fibroblast in vitro]
Jie Zhang1, Ping Lin, Lu-gang Huang
1Institute of Cancer, West China Hospital, Sichuan University, Chengdu, Sichuan, P. R. China 610041.
Objective:
To explore the autocrine regulation for the transforming growth factor-beta 1(TGF-beta 1) by the fibroblasts in vitro.
Methods:
Fibroblasts were cultured in vitro which isolated from the normal prepuce by circumcision. TGF-beta 1 concentration were determined with ELISA for the different passages and the different time at the same passage.
Results:
The TGF-beta 1 concentration was different for the distinct passage of fibroblasts and achieved the peak (450 ng/L) at the sixth passage. Among the same fibroblasts, the TGF-beta 1 concentration was different in the various days and passage of the summit (680 ng/L) on the fifth day, which was 2.5 times as high as that of the first day.
Conclusion:
The fibroblasts possess the ability of autocrine of the TGF-beta 1 and have some regularity. It will provide the theory basis for the research about TGF-beta 1 regulation mechanism and the medical application about salvia miltiorrhiza.