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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Trends in mass spectrometry instrumentation for proteomics
1Environmental & Molecular Sciences Laboratory, Pacific Northwest National Laboratory RO. Box 999, MSIN: K8-98, Richland, WA 99352, USA.
Trends in Biotechnology
|February 7, 2003
Summary
Mass spectrometry is a key tool for proteomics, enabling rapid protein identification and quantification. Future advancements promise significant improvements in sensitivity, dynamic range, and throughput for proteomic analyses.
Area of Science:
- Proteomics
- Analytical Chemistry
- Biochemistry
Background:
- Mass spectrometry (MS) is a cornerstone technology in proteomics, offering rapid and sensitive protein identification and quantification capabilities.
- Current MS techniques allow for the identification of thousands of proteins from microgram samples within a day and relative protein abundance quantification.
Purpose of the Study:
- To highlight the immense need for enhanced proteome measurement capabilities.
- To discuss new strategies and instrument advancements driving the field forward.
Main Methods:
- Integration of multi-dimensional liquid separations with mass spectrometry.
- Utilization of high-accuracy mass measurements for enhanced proteomic analyses.
Main Results:
- Current MS enables high-throughput protein identification and quantification from small sample amounts.
- Emerging strategies and instrument advances are poised to significantly improve proteomic analysis.
Conclusions:
- Ongoing developments in MS, including hyphenation with advanced separation techniques and high-accuracy measurements, are expected to yield substantial improvements.
- These advancements promise >10-fold increases in sensitivity, dynamic range, and throughput for future proteomic studies.
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