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Progressive decrease of intramyocellular accumulation of H+ and Pi in human skeletal muscle during repeated isotonic

J Rico-Sanz1

  • 1Copenhagen Muscle Research Center, The Panum Institute, University of Copenhagen, Copenhagen DK-2100, Denmark. rico-sj@pbrc.edu

American Journal of Physiology. Cell Physiology
|February 7, 2003
PubMed
Summary

Repeated intense exercise did not cause muscle fatigue due to high energy phosphate depletion or accumulation of hydrogen ions and inorganic phosphate (Pi). These findings challenge previous hypotheses on exercise-induced muscle fatigue mechanisms.

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Area of Science:

  • Exercise Physiology
  • Biochemistry
  • Muscle Metabolism

Background:

  • Understanding the metabolic factors contributing to skeletal muscle fatigue is crucial for optimizing athletic performance and training.
  • Previous hypotheses suggested that the accumulation of hydrogen ions (H+) and inorganic phosphate (Pi) could be primary drivers of fatigue during intense exercise.

Purpose of the Study:

  • To investigate whether repeated bouts of isotonic exercise lead to an accumulation of hydrogen ions and/or inorganic phosphate (Pi) in skeletal muscle.
  • To test the hypothesis that these metabolic changes contribute to exercise-induced muscle fatigue.

Main Methods:

  • Utilized phosphorus-31 Magnetic Resonance Spectroscopy ((31)P-MRS) to non-invasively assess muscle metabolites in the gastrocnemius of trained female athletes.

Related Experiment Videos

  • Subjects performed four repeated bouts of isotonic plantar flexion exercise, each separated by a short rest period.

    • Main Results:

    • Muscle adenosine triphosphate (ATP) levels remained stable throughout the exercise bouts.
    • Phosphocreatine (PCr) degradation was highest during the first exercise bout and decreased with subsequent bouts.
    • Intramyocellular pH, total Pi, and various protonated forms of Pi were significantly higher at the end of the first exercise bout compared to later bouts.
    • Rating of perceived exertion increased progressively with each exercise bout.

    Conclusions:

    • Muscle fatigue during repeated intense isotonic exercise is not caused by the progressive depletion of high-energy phosphates (ATP and PCr).
    • Intracellular accumulation of hydrogen ions, total Pi, monoprotonated Pi, or diprotonated Pi does not appear to be the primary cause of fatigue in this exercise model.
    • These findings suggest alternative mechanisms contributing to muscle fatigue during repeated strenuous exercise.