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Updated: Sep 27, 2026
Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection
Published on: June 12, 2014
[Preliminary study on isolation, purification and hydrolytic activity of cysteine proteinases in Entamoeba
Objective:
To explore the invading mechanism of amebae in lamina porpria and observe the interaction between the cysteine proteinase (CP) of Entamoeba histolytica and laminin.
Methods:
CP was identified by laminin-sepharose affinity chromatography, followed by isolation, purification and inhibitor experiment. The hydrolytic activity was measured by gelatin electrophoresis.
Results:
Purified CP of E. histolytica showed a strong affinity with laminin. The molecular weight of CP is 27 kDa. It can be inhibited by EC-64 and exhibited a protein hydrolytic activity.
Conclusion:
The specific affinity and hydrolytic activity of CP might play an important role in its invasion to the basement membrane of intestinal mucosa.
