Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tau could protect DNA double helix structure.

Qian Hua1, Rong-qiao He

  • 1Laboratory of Visual Information Processing, Center for Brain and Cognitive Sciences, Institute of Biophysics, The Chinese Academy of Sciences, 15 Da Tun Road, Chaoyang District, Beijing 100101, PR China.

Biochimica Et Biophysica Acta
|February 8, 2003
PubMed
Summary

Tau protein stabilizes double-stranded DNA against heat-induced denaturation and enhances its renaturation. It also protects DNA from hydroxyl radical damage, indicating a protective role for DNA.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association between hemoglobin-to-red cell distribution width ratio and occurrence of sepsis during ICU stay in inflammatory bowel disease patients who died: a retrospective study using the EICU-CRD database.

PloS one·2026
Same author

Functional Suppression of SCAP Triggers Endoplasmic Reticulum Stress-Dependent Ferroptosis by Impairing Cholesterol Metabolism in Gastric Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Kinsenoside Targets IDH1 to Restore Microglial Immune-Metabolic Homeostasis for Alzheimer's Disease Therapy.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

18ï ¢-Glycyrrhetinic acid suppresses glioblastoma by regulating p38 signaling pathway: an integrative approach combining network analysis, transcriptomics, and experimental assessment.

Frontiers in pharmacology·2026
Same author

The Radioprotective Effects of (-)-Epigallocatechin-3-gallate (EGCG): Restoring the Gut Microbiota-Immune Axis to Ameliorate Radiation-Induced Intestinal Injury.

Dose-response : a publication of International Hormesis Society·2026
Same author

Inflammatory Factors Derived From Metabolic Dysfunction-Alcoholic Fatty Liver Disease: Inducers of Anxiety and Spatial Memory Impairment.

Mediators of inflammation·2026

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • The hyperchromic effect is a method to study DNA denaturation.
  • Tau protein's interaction with DNA is not fully understood.

Purpose of the Study:

  • To investigate the effect of tau protein on DNA stability and denaturation.
  • To determine if tau protein protects DNA from damage.

Main Methods:

  • Hyperchromic effect analysis using UV-Vis spectrophotometry.
  • Thermal denaturation studies of DNA with and without tau.
  • Circular dichroism spectroscopy.
  • Hydroxyl radical protection assays.

Main Results:

  • Tau increased DNA melting temperatures (calf thymus DNA: 67°C to 81°C; plasmid DNA: 75°C to 85°C).

Related Experiment Videos

  • Tau slowed DNA denaturation but accelerated renaturation.
  • Minimal conformational changes in DNA double helix were observed with tau.
  • Tau protected DNA from hydroxyl radical-induced damage in vitro.

    • Conclusions:

    • Tau protein stabilizes DNA against thermal denaturation and promotes renaturation.
    • Tau acts as a DNA-protecting molecule against radical damage.
    • Tau does not significantly alter DNA double helix conformation.