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Related Experiment Videos

Liquid-liquid immiscibility in membranes.

Harden M McConnell1, Marija Vrljic

  • 1Department of Chemistry, Biophysics Program, Stanford University, Stanford, California 94305-5080, USA. harden@stanford.edu

Annual Review of Biophysics and Biomolecular Structure
|February 8, 2003
PubMed
Summary
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Cholesterol and phospholipid mixtures show liquid-liquid immiscibility, forming "condensed complexes." This phase separation is influenced by membrane composition and temperature, impacting cell membrane biophysics.

Area of Science:

  • Membrane biophysics and biochemistry
  • Physical chemistry of lipid mixtures

Background:

  • Cholesterol-phospholipid mixtures exhibit liquid-liquid immiscibility.
  • Some mixtures display multiple immiscibility phenomena.

Purpose of the Study:

  • To explore the physical chemistry of cholesterol-phospholipid mixtures.
  • To develop a thermodynamic model for observed immiscibilities.

Main Methods:

  • Thermodynamic modeling of condensed complexes.
  • Analysis of phase separation in lipid mixtures.

Main Results:

  • A thermodynamic model of "condensed complexes" was developed.
  • These complexes form due to an exothermic reaction between cholesterol and phospholipids.

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  • Phase separation into a complex-rich phase is dependent on membrane composition and temperature.

    • Conclusions:

    • Defined cholesterol-phospholipid mixtures offer a model for understanding animal cell membrane biophysics and biochemistry.
    • The condensed complexes model explains phase separation phenomena in lipid bilayers.