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Updated: Aug 8, 2026
qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
Published on: July 10, 2014
Detection of Candida albicans mRNA from formalin-fixed, paraffin-embedded mouse tissues by nested reverse
David A Schofield1, Caroline Westwater, Emily E Paulling
1Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina 29403, USA. schofida@musc.edu
Abstract:
Histopathology archives represent a vast source of infectious disease specimens that can be used to elucidate important disease processes. In this report, we describe a method to detect Candida albicans gene expression from infected, formalin-fixed, paraffin-embedded mouse tissue. By use of glass beads to break open the fungal cells and proteinase K treatment, RNA was extracted routinely from tissue sections that had been fixed for up to 72 h. Upon reverse transcription of the RNA and nested PCR, the procedure detected C. albicans "housekeeping" and putative virulence genes.
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