Development of a 5' fluorogenic nuclease-based real-time PCR assay for quantitative detection of Actinobacillus

Related Experiment Video

Updated: Sep 27, 2026

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Akihiro Yoshida1, Nao Suzuki, Yoshio Nakano

  • 1Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan.

Journal of Clinical Microbiology
|February 8, 2003
PubMed

More Related Videos

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings09:36

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...