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Related Experiment Videos

H2-M3-restricted memory T cells: persistence and activation without expansion.

Kristen M Kerksiek1, Alexander Ploss, Ingrid Leiner

  • 1Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|February 8, 2003
PubMed
Summary

H2-M3-restricted T cells show faster primary responses to Listeria monocytogenes infection. Despite similar memory markers, H2-M3 T cells exhibit distinct reinfection proliferative programs compared to MHC class Ia-restricted T cells.

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Area of Science:

  • Immunology
  • Microbial Pathogenesis

Background:

  • Conventional T cell responses are mediated by MHC class Ia molecules.
  • H2-M3-restricted T cells offer a distinct pathway for immune surveillance.
  • Listeria monocytogenes infection serves as a model for studying T cell responses.

Purpose of the Study:

  • To investigate the differential responses of H2-M3-restricted and MHC class Ia-restricted T cells during primary and secondary Listeria monocytogenes infection.
  • To characterize the generation and function of memory T cells in H2-M3-restricted populations.

Main Methods:

  • Comparative analysis of T cell proliferation and activation markers following primary and secondary L. monocytogenes infection.
  • Flow cytometry to assess cell surface memory markers.
  • In vitro priming experiments using L. monocytogenes-derived peptides and co-stimulatory signals.

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Main Results:

  • H2-M3-restricted T cells respond more rapidly during primary infection than MHC class Ia-restricted T cells.
  • Secondary infection leads to robust expansion of H2-K(d)-restricted T cells but not H2-M3-restricted CTLs.
  • H2-M3-restricted memory T cells are generated, sharing surface markers and longevity with classical memory T cells.
  • Priming strategies can induce H2-M3-restricted memory T cells capable of antigen-specific expansion upon secondary exposure.

Conclusions:

  • Disparate memory T cell responses are attributed to intrinsic differences between H2-M3 and MHC class Ia-restricted T cell populations.
  • While initial proliferative programs differ, H2-M3-restricted T cells can retain the capacity for expansion under specific inflammatory conditions.