[Determination of resibufogenin in xinli pills by RP-HPLC]

A method has been developed for the quantitative determination of Resibufogenin in Xinli pills by RP-HPLC. The stainless steel column(200 x 4.6 mm) was packed with ODS-3 (particle size 5 microns). The mobile phase was a mixture of methanol-water (60:40). The flow rate was 1 ml/min. The column temperature was 30 degrees C. The detection wavelength was 299 nm. The retention time for recibufogenin was 5.6 min. The average recovery for resibufogenin was 99.9%. The linear calibration curve for resibufogenin was obtained in the range 53.0-159.0 micrograms with the correlation coefficient 0.9998.