Related Experiment Videos
[Osteocalcin and type I collagen mRNA expression of rat marrow stromal cells under induction condition]
Huaiqing Chen1, Jun Han, Liang Li
1Institute of Biomedical Engineering, West China Medical Center, Sichuan University, Chengdu 610041, China.
Objective:
To investigate osteocalcin and type I collagen mRNA expression of rat marrow stromal cells under induction condition.
Methods:
Cultured rat marrow stromal cells were induced by osteogenic compound(composed of dexamethasone 10(-3) mol/L, beta glyceralphosphate 10 mmol/L, ascorbic acid 50 micrograms/ml). Osteocalcin and type I collagen mRNA expression were measured using RT-PCR technique.
Results:
It was found that rat marrow stromal cells in induced group have an increased time-dependent expression of osteocalcin and type I collagen mRNA, in comparison with control groups. And the necessity of 1,25-(OH)2D3 in induced osteocalcin mRNA expression was confirmed.
Conclusion:
The cultured rat marrow stromal cells kept the undifferentiated state and had osteogenic potential testified by increased osteocalcin and type I collagen mRNA expression when induced.