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[Study on the expression of adhesion molecule related proteins in patients with coronary heart disease]
Xiaoping Chen1, Dingbao Zhang, Qing Yang
1Department of Cardiology, West China Hospital, Sichuan University, Chengdu 610041, China.
Insights
Coronary heart disease patients, especially those with unstable angina, show increased CD11b adhesion molecules on neutrophils and lymphocytes. However, CD11b levels do not correlate with coronary artery stenosis severity.
Area of Science:
- Immunology
- Cardiology
- Biochemistry
Background:
- Coronary heart disease (CHD) involves complex inflammatory processes.
- Adhesion molecules play a role in inflammatory cell trafficking.
Purpose of the Study:
- To evaluate CD11a and CD11b expression on immune cells in CHD patients.
- To correlate expression levels with coronary artery stenosis and disease severity.
Main Methods:
- Fluorescent immunoassay on flow cytometry used to measure CD11a and CD11b.
- Subjects categorized into stable angina, unstable angina, and healthy controls.
Main Results:
- CD11b expression was significantly higher on lymphocytes and neutrophils in stable and unstable angina groups compared to controls.
- No significant difference in CD11a expression was observed between groups.
- Increased CD11b in severe stenosis was not statistically significant.
Conclusions:
- Elevated CD11b on neutrophils and lymphocytes is associated with unstable angina.
- CD11b expression levels do not correlate with the degree of coronary artery stenosis.
Objective:
To assess the expression level of adhesion molecule related proteins CD11a and CD11b on neutrophils, monocytes and lymphocytes in patients with coronary heart disease and detect the relation of expression level to the degree of stenosis of coronary arteries and the severity of coronary heart disease.
Methods:
The subjects were divided into three groups: stable angina, unstable angina, and healthy people as control. The amount of cell adhesion molecule related proteins CD11a and CD11b on neutrophilic granulocytes, monocytes and lymphocytes of peripheral blood was measured in three groups by using fluorescent immunoassay method on flow cytometer. Relationship between them was analysed.
Results:
No significant difference in the expression of CD11a between the angina and control groups was observed. The expression of CD11b on lymphocytes and neutrophilic granulocytes in the unstable angina group and stable angina group was significantly higher than that in the healthy people(P < 0.01 and P < 0.05). Although the amount of CD11b measured in the group of severe coronary artery stenosis was higher than that measured in the mild light group, the difference was not significant (P > 0.05).
Conclusion:
The amount of adhesion molecule related protein CD11b on neutrophilic granulocytes and lymphocytes in patients with unstable angina is higher than that in patients with stable angina and control group, but there is no relationship between the expression level of CD11b and the degree of coronary stenosis.