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Updated: Jun 27, 2026

Culturing Primary Rat Inner Medullary Collecting Duct Cells
Published on: June 21, 2013
[Specificity of gene di (diabetes insipidus) expression in homologous inbred rat strains]
1Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090 Russia. khegay@bionet.nsc.ru
Abstract:
The diabetes insipidus mutation is displayed in homozygotes in the form of diabetes insipidus with water consumption from 30 to 100% of body weight per day. We developed two inbred sublines of the di/di Brattleboro rats as well as the recombinant inbred subline by integrating genes of August rats into the di/di mutant genome. Changes in the genetic background proved to have no effect on the quantitative parameters of the diabetes insipidus. The intensity of the secondary immune response and the content of tropomyosin in the medulla of the rat kidney can serve as additional marker traits of the di/di genotype.
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