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Sodium crocetinate does not alter gut hypercapnic responses or renal energy stores during transient sub-diaphragmatic
Thomas J Morgan1, Balasubramanian Venkatesh, Agnieszka Crerar-Gilbert
1Adult Intensive Care, Mater Misericordiae Health Services, Raymond Terrace, 4101, South Brisbane, Queensland, Australia. thomas_morgan@mater.org.au
Objectives:
To evaluate the protection afforded by trans-sodium crocetinate against dysoxia in an animal model of recurrent sub-diaphragmatic ischaemia.
Design:
Prospective experimental animal study.
Setting:
University research laboratory
Subjects:
Adult male Sprague-Dawley rats.
Interventions:
Twelve adult male Sprague-Dawley rats (340-510 g) were anaesthetised with sodium pentobarbitone 60 mg/kg i.p. and ventilated with oxygen and isoflurane via tracheostomy. Six 2-min episodes of sub-diaphragmatic hypotension (mean pressure 30 mmHg) were induced using a sling around the proximal aorta. Before the third and sixth episodes, saline 1.5 ml/kg was injected into the aortic cannula. In six rats, this saline contained trans-sodium crocetinate 50 microg/ml.
Measurements And Main Results:
Ileal luminal PCO(2) and distal aortic pressure were monitored continuously. Following ischaemic episodes trans-sodium crocetinate had no discernible effect on either degree of PCO(2) elevation or the time to peak PCO(2). No effects on renal energy charge or nucleotide concentrations were detected. UV-visible spectroscopy of the crocetinate preparation showed that some cis isomer was present.
Conclusions:
The findings, although limited to one drug dosage in one animal model, bring into question whether trans-sodium crocetinate affects plasma oxygen diffusivity in vivo. Alternative explanations for the negative findings include a TSC-induced exacerbation of arterio-venous oxygen shunting, the brevity of the dysoxic episodes, and the presence of cis isomer.