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[Experimental study of inhibitory effect of kangxian recipe on TGF-beta 1 induced hepatocyte apoptosis]
1First Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou 310003.
Objective:
To study the inhibitory effect of Kangxian Recipe (KXR) on TGF-beta 1 induced hepatocyte apoptosis.
Methods:
The in vitro model of hepatocyte apoptosis was established by cell biologic methods, utilizing the characteristics of TGF-beta 1 to observe the inhibitory effect of KXR on hepatocyte apoptosis.
Results:
TGF-beta 1 induced apoptosis of hepatocyte in a dose-dependent manner. The apoptosis rate of 2.2.15 cells was 63% when 500 ng/L TGF-beta 1 was used, while for HepG2 cell, it was merely 44%. After treatment of 20 ng/L KXR, the apoptosis rate of the two kinds of cell lines lowered to 33% and 24% respectively. The inhibition rate of both groups was about 50%.
Conclusion:
KXR had strong inhibitory effect on hepatocyte apoptosis induced by TGF-beta 1.
