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Elimination of the sarcoma genome from murine sarcoma virus transformed cat cells

A S Frankel, D K Haapala, R L Neubauer

    Science (New York, N.Y.)
    |March 26, 1976
    PubMed

    Insights

    Spontaneous revertant cat cell lines lost the Moloney murine sarcoma virus genome. This study reports the first evidence of transforming genome loss in revertant cell lines, confirmed by hybridization techniques.

    Area of Science:

    • Molecular Biology
    • Virology
    • Cell Biology

    Background:

    • Moloney murine sarcoma virus (MMSV) infection induces malignant transformation in cat cells.
    • Transformed cells harbor virus-specific genetic material (RNA and DNA).

    Purpose of the Study:

    • To investigate the genetic stability of transformed cat cells.
    • To determine if spontaneously reverting cell lines retain the sarcoma genome.

    Main Methods:

    • Culturing of MMSV-transformed cat cells.
    • Isolation and cloning of spontaneous revertant cell lines.
    • RNA-complementary DNA (cDNA) and DNA-complementary DNA (cDNA) hybridization assays to detect viral sequences.

    Main Results:

    • Transformed cat cells contained virus-specific sequences in both RNA and DNA.
    • Spontaneous revertant cell lines showed no detectable evidence of the sarcoma genome.
    • Hybridization assays confirmed the absence of viral genetic material in revertant cells.

    Conclusions:

    • Revertant cat cell lines derived from MMSV-transformed cells have lost the transforming sarcoma genome.
    • This study provides the first report of a transforming genome being lost in a revertant cell line.

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