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Purification of lysozyme from other hen's-egg-white proteins using metal-affinity precipitation
Ipsita Roy1, Mukkavilli V S Rao, Munishwar N Gupta
1Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.
Biotechnology and Applied Biochemistry
|February 13, 2003
Abstract:
It was found that the presence of 5 mM Cu(2+) caused precipitation of protein present in hen's egg white to a large extent. About 85% of lysozyme activity remained in the supernatant and the enzyme was purified by approx. 13-fold. A further gel-filtration step on Sephadex G-75 resulted in an overall yield of 80% for the enzyme with 655-fold purification, and showed a single band on SDS/PAGE.