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Updated: Sep 27, 2026
Ex Vivo Corneal Organ Culture Model for Wound Healing Studies
Published on: February 15, 2019
[mRNA expression of keratoepithelin in cornea after photorefractive keratectomy in rabbits]
1Department of Ophthalmology, Xiangya Hospital, Zhongnan University, Changsha, 410078, China.
Objective:
To investigate the roles of keratoepithelin(KE) during healing after photorefractive keratectomy (PRK).
Methods:
20 of 24 rabbits underwent bilateral 193 nm excimer laser photorefractive keratectomy to correct 10 diopters of myopia, and the other 4 rabbits as normal group. The mRNA levels of KE in corneas of the rabbits were determined by the in situ hybridization technique with a Digoxigenin labled probe.
Results:
In normal group, there was no mRNA expression of KE in cornea. on 7, 14 days after operation, mRNA expression of KE was observed in epithelium and stroma of operation group, while on 28 days mRNA expression of KE was observed only in epithelium.
Conclusion:
KE was involved in the synthesis of extracellular matrix during healing after PRK and may play an important role in the development of haze by maintaining proper spacing among collagen fibrils.
