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[SFE-CGC determination of tanshinone in Salvia miltiorrhiza Bunge]
1School of Pharmacy, Second Military Medical University, Shanghai 200433, China. chenbin-sh@hotmail.com
Aim:
To analyze tanshinone IIA in Salvia miltiorrhiza Bunge by supercritical fluid extraction (SFE) coupled with capillary gas chromatography (CGC).
Methods:
The solution pressure was predicted by solubility parameter theory, the extraction conditions were confirmed by orthogonal design in which temperature, volume of modifier and dynamic extracting volume had been studied in three levels.
Results:
This method is quick, convenient, precise and accurate, added sample recovery is 95.3%, RSD = 4.27%.
Conclusion:
Compared with the method of ultrasonic extraction, significantly better qualitative and quantitative result could be obtained by SFE-CGC and expend less organic solvent then make less pollution.
