[Analysis of the metabolite of 7-(4-chlorbenzyl)-7,8,13,13a-tetrahydroberberine in rabbit]
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1China Pharmaceutical University, Nanjing 210009, China. npfeng@citiz.net
Aim:
To explore the biotransformation of compound 7-(4-chlorbenzyl)-7,8,13, 13a-tetrahydroberberine in the rabbit.
Methods:
Analyze the rabbit bile sample with HPLC, LC/MS and LC/NMR.
Results:
A metabolite and unchanged 7-(4-chlorbenzyl)-7,8,13,13a-tetrahydroberberine were found in the rabit bile, the metabolite was characterized and its structure was elucidated.
Conclusion:
Compound 7-(4-chlorbenzyl)-7,8,13,13a-tetrahydroberberine is metabolized by demethylation at 10-OCH3 position.
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