Related Experiment Video
Updated: Aug 5, 2026
A Step by Step Protocol for Subretinal Surgery in Rabbits
Published on: September 13, 2016
[Effect of amniotic membrane on filtering bleb after trabeculectomy in rabbit eyes]
1Ophthalmic Center, Ruijin Hospital, Shanghai Second Medical University, Shanghai, 200025 China.
Objective:
To investigate the effects of amniotic membrane on filtering blebs after trabeculectomy.
Methods:
Twelve rabbits underwent bilateral trabeculectomies. One eye was randomly sutured preserved human amniotic membrane between the sclera and the scleral flap and the other eye served as the control. The filtering blebs and filtering function were observed at the 1st, 2nd and 3rd week after the operation. The scar development situation between bleb and episclera was observed under the light microscope.
Results:
The blebs in amniotic membrane group were eminence, effuse and had a good filtering function at the 1st, 2nd and 3rd week after the operation. The blebs in control group were cicatrized and had a bad filtering function. Histological observation showed no scar development, less fibroblast cell and more inflammatory cells infiltration at the site of trabeculectomy in amniotic membrane group. On the contrary, the control group showed more fibroplasia and fibroblast hyperplasia.
Conclusion:
Amniotic membrane can reduce the scar, improve filtering function.
More Related Videos
11:03Subretinal Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells into a Large-eyed Model of Geographic Atrophy
Published on: January 22, 2018
07:10Ex Vivo and In Vivo Animal Models for Mechanical and Chemical Injuries of Corneal Epithelium
Published on: April 6, 2022