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Updated: Sep 27, 2026
Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
Published on: June 10, 2020
[Evaluation of polymerase chain reaction for detection of chlamydia trachomatis in eye swabs]
Objective:
To investigate the clinical value of Polymerase Chain Reaction (PCR) in the diagnosis of trachoma.
Methods:
Direct amplication of 16S rRNA gene which is chlamydia-specific by PCR was used to detect chlamydia trachomatis (CT).
Results:
Of 93 subjects who conformed to the diagnosis criteria of trachoma, 69 (74.2%) was PCR-Positive; Of 178 subjects who did not comform to the criteia, 6(3.4%) was PCR-positive. PCR had a sensitivity of 74.2% and a speciticity of 96.7% for the clinical diagnosis of trachoma. The difference between trachoma group and nontrachoma group is significant(P < 0.001).
Conclusion:
The PCR may be a good method in the diagnosis of ocular chlamydia trachomatis infection because of its excellent sensitivity and specificity.
