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[Analysis and structure identification of trace constituent in the total ginkgolide by using LC/DAD/ESI/MS]
1Analytical Chemistry Center, China Pharmaceutical University, Nanjing 210009, China. km4ywang@sina.com
Aim:
To identify the trace constituents in the total ginkgolide from leaves of Ginkgo biloba.
Methods:
The extract was analyzed by using LC/DAD/ESI/MS combination technology, both UV and mass spectrums of each compound were recorded simultaneously. UV, IR, MS and NMR spectra were employed for structural identification.
Results:
With higher sensitivity of the MS detector, three trace constituents were found as well as the known compounds ginkgolide A (GA), ginkgolide B (GB), ginkgolide C (GC) and bilobalide (BD).
Conclusion:
Two compounds (tR 57.8 and 56.7 min) were identified as 1, 10-dihydroxy-3, 14-didehydroginkgolide and 10-hydroxy-3, 14-didehydroginkgolide, named as ginkgolide K and ginkgolide L, respectively, according to the characteristics of their UV, MS, IR and NMR spectra. It is the first time of finding the ginkgolide compounds containing double bond.
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