Related Experiment Video
Updated: Sep 27, 2026
Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
Published on: April 17, 2011
[The preliminary experiment of gene transfer into cultured rat retinal neural cells]
1Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Purpose:
To investigate the possibility of gene transfer into retina neural cells.
Methods:
pcDNA3-LacZ and pcDNA3-GDNF were transferred into rat retinal neural cells with cationic lipofectin. The gene transfer and expression of GDNF were detected by means of RT-PCR and SDS-PAGE.
Results:
pcDNA3-LacZ and pcDNA3-GDNF could be transferred into retinal neural cells and the expressive product of GDNF gene, a 32 KD protein band, was detected.
Conclusion:
The preliminary results suggest that LacZ gene could be a marker to identify gene transfer into the target tissue and cells; GDNF gene could be transferred into cultured retinal neural cells of SD rats, and express 32 KD protein.
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