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Updated: Jul 23, 2026

Microscopic Observation of Lymphocyte Dynamics in Rat Peyer's Patches
Published on: June 25, 2020
[The observation of proliferative cell nuclear antigen and AgNORs in ocular adnexal lymphoid hyperplasia]
1Department of Ophthalmology, Changzheng Hospital, Second Military University, Shanghai 200003, China.
Purpose:
To investigate the expression of proliferative cell nuclear antigen and Nuclear Organizer Region in ocular adnexal lymphoid hyperplasia and its histopathologic classification.
Method:
Using anti-proliferative cell nuclear antigen monoclonal antibody (PC10) and AgNORs method, the proliferative cell in 34 cases of ocular adnexal lymphoid neoplasms (benign lymphoid lesion, BLL, 14 cases; atypical lymphoid hyperplasias, AH, 9 cases; malignant lymphomas, ML, 11 cases) were detected in formalin fixed and paraffin-embeded tissue.
Results:
A significant difference between BLL and AH, BLL and ML was observed (PCNA P < 0.001, P < 0.001; AgNORs P < 0.01, P < 0.001); similar result was observed between AH and ML in PCNA index (P < 0.01). But there was not a significant difference in AgNORs count (P > 0.05). The linear regression coefficient between PCNA index and AgNORs number was excellent (0.8693).
Conclusion:
The two methods may be helpful to distinguish ocular adnexal lymphoid reactive hyperplasia and malignant lymphoma.
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