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Salicylic acid inhibits indeterminate-type nodulation but not determinate-type nodulation.

Paulina C van Spronsen1, Teun Tak, Anita M M Rood

  • 1Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands. spronsen@rulbim.leidenuniv.nl

Molecular Plant-Microbe Interactions : MPMI
|February 13, 2003
PubMed
Summary
This summary is machine-generated.

Salicylic acid (SA) blocks nodulation in indeterminate-type plants like vetch by interfering with lipochitin oligosaccharides (LCOs) signaling. This suggests SA can differentiate signaling pathways between plant nodulation types.

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Area of Science:

  • Plant-microbe interactions
  • Molecular signaling in legumes
  • Plant physiology

Background:

  • Lipochitin oligosaccharides (LCOs), or Nod factors, mediate symbiotic signaling between rhizobia and legumes.
  • The acyl chain structure of LCOs, specifically 18:4 vs. 18:1, influences nodulation in different plant types.
  • Salicylic acid (SA) is known to modulate plant signaling pathways, including those involving oxylipins.

Purpose of the Study:

  • To investigate the role of salicylic acid (SA) in the nodulation process of Vicia sativa subsp. nigra (vetch), an indeterminate-type nodulating plant.
  • To determine if SA interferes with LCO-mediated signaling, particularly the mitogenic effects of 18:4 LCOs.
  • To compare the effects of SA on nodulation in indeterminate-type versus determinate-type nodulating plants.

Main Methods:

  • Application of 10(-4) M salicylic acid (SA) to Vicia sativa subsp. nigra (vetch) and Lotus japonicus plants during rhizobial inoculation.
  • Assessment of nodulation, root-hair deformation, and mitogenic responses to LCOs in the presence of SA.
  • Testing the effect of SA on association between rhizobia and plant roots.

Main Results:

  • SA (10(-4) M) completely blocked nodulation in vetch and inhibited the mitogenic effect of 18:4 LCOs, but not root-hair deformation.
  • SA also inhibited the association of Rhizobium leguminosarum bv. viciae with vetch roots.
  • SA did not inhibit nodulation in Lotus japonicus (determinate-type) or other determinate-type plants, but did inhibit other indeterminate-type plants.

Conclusions:

  • The 18:4 acyl chain on LCOs is crucial for signaling in indeterminate-type nodulation, and SA interferes with this pathway.
  • SA's inhibitory effect is specific to indeterminate-type nodulating plants, suggesting distinct signal transduction mechanisms.
  • SA serves as a valuable tool for dissecting the differences in signaling pathways between indeterminate- and determinate-type nodulating legumes.