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Related Experiment Videos

Rat strain differences in peripheral and central serotonin transporter protein expression and function.

Francesca Fernandez1, Sophie Sarre, Jean-Marie Launay

  • 1NeuroGénétique et Stress, INSERM U471-INRA UR502, Institut F. Magendie, Rue Camille Saint Saëns, 33077 Bordeaux Cédex, France.

The European Journal of Neuroscience
|February 13, 2003
PubMed
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Fischer 344 rats exhibit higher serotonin transporter (5-HTT) function centrally and peripherally compared to Lewis rats. These differences, not in coding sequences, suggest F344 and LEW rats may model human 5-HTT promoter gene variations.

Area of Science:

  • Neuroscience
  • Pharmacology
  • Genetics

Background:

  • Female Fischer 344 (F344) rats show increased serotonin transporter (5-HTT) gene expression in the dorsal raphe compared to Lewis (LEW) rats.
  • Previous studies indicate potential strain-specific differences in 5-HTT expression and function.

Purpose of the Study:

  • To investigate central and peripheral 5-HTT protein expression and function differences between F344 and LEW rat strains.
  • To determine if these strain differences are influenced by the hypothalamo-pituitary-adrenal (HPA) axis.

Main Methods:

  • Synaptosomal preparations and in vivo microdialysis were employed to assess 5-HTT binding and serotonin (5-HT) reuptake.
  • Blood platelet 5-HTT expression and function were also analyzed.
  • Saturation binding studies determined Bmax and Vmax values for 5-HTT binding and reuptake.

Related Experiment Videos

  • HPA axis manipulations were performed to assess the role of corticosteroids.

    • Main Results:

    • F344 rats, both male and female, displayed increased midbrain and hippocampal 5-HTT binding and hippocampal 5-HT reuptake compared to LEW rats.
    • These strain differences extended to blood platelet 5-HTT expression and function in homebred rats.
    • HPA axis manipulation did not account for the observed strain differences in hippocampal 5-HTT binding and reuptake.
    • Hippocampal extracellular 5-HT levels were lower in F344 rats, with a larger relative increase upon citalopram administration.

    Conclusions:

    • Significant strain differences in central and peripheral 5-HTT protein expression and function exist between F344 and LEW rats.
    • These differences are not located in the 5-HTT gene's coding sequences.
    • F344 and LEW rat strains may serve as valuable models for studying functional polymorphisms in the promoter region of the human 5-HTT gene.