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Related Experiment Videos

The local lymph node assay: past, present and future.

Ian Kimber1, R J Dearman, D A Basketter

  • 1Syngenta Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, UK. ian.kimber@syngenta.com

Contact Dermatitis
|February 13, 2003
PubMed
Summary
This summary is machine-generated.

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The local lymph node assay (LLNA) identifies skin sensitizers by measuring lymphocyte proliferation. This validated method is now used for assessing chemical potency and risk assessment in humans.

Area of Science:

  • Toxicology
  • Immunology
  • Dermatology

Background:

  • The local lymph node assay (LLNA) was developed to identify chemicals causing skin sensitization and allergic contact dermatitis.
  • It relies on lymphocyte proliferation in lymph nodes stimulated by chemical allergens.

Purpose of the Study:

  • To review the development, validation, and application of the LLNA for hazard identification.
  • To discuss the LLNA's use in assessing contact allergen potency and risk assessment.
  • To explore potential new applications and modifications of the LLNA.

Main Methods:

  • The LLNA measures lymphocyte proliferation in lymph node cells (LNC).
  • Chemicals inducing a 3-fold or greater increase in LNC proliferation compared to controls are classified as sensitizers.

Related Experiment Videos

  • Potency is determined by the EC3 value, the concentration causing a 3-fold LNC proliferation increase.

    • Main Results:

    • The LLNA is a validated, stand-alone test for hazard identification of skin sensitizers.
    • The EC3 value derived from LLNA correlates well with human data on skin sensitizer potency.
    • The LLNA is increasingly used for risk assessment of contact allergens.

    Conclusions:

    • The LLNA is a reliable method for both hazard identification and potency assessment of skin sensitizers.
    • Its application in risk assessment is supported by its correlation with human data.
    • Ongoing research explores new uses and modifications for the LLNA.