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Identification of a novel insertion sequence in vanB2-containing Enterococcus faecium.

W G Lee1, W Kim

  • 1Department of Clinical Pathology and Institute for Medical Sciences, Ajou University School of Medicine, Suwon, South Korea. weegyo@ajou.ac.kr

Letters in Applied Microbiology
|February 13, 2003
PubMed
Summary

A novel insertion sequence, ISEnfa3, was identified in vanB2-containing Enterococcus faecium. This ISEnfa3 sequence can serve as a valuable tool for tracking the spread of vanB2 resistance in Enterococcus faecium strains.

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Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Enterococcus faecium is a significant opportunistic pathogen.
  • The vanB2 gene confers resistance to vancomycin, a critical antibiotic.
  • Understanding the genetic elements associated with vanB2 in E. faecium is crucial for infection control.

Purpose of the Study:

  • To characterize a novel insertion sequence (IS) found in vanB2-containing Enterococcus faecium.
  • To determine the genetic features and potential function of this new IS element.

Main Methods:

  • Direct PCR amplification of the ORFC region of Tn5382 from vanB2-positive E. faecium DNA.
  • DNA sequencing and in silico analysis to identify and characterize the insertion sequence.

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Main Results:

  • A novel insertion sequence, designated ISEnfa3, was identified.
  • ISEnfa3 is 1418 bp long with a putative transposase gene.
  • It possesses 12 bp inverted terminal repeats but lacks direct repeats.

Conclusions:

  • ISEnfa3 shows high similarity to transposases of IS3 family members.
  • The consistent presence of ISEnfa3 in vanB2-containing E. faecium strains suggests its potential utility.
  • ISEnfa3 can be utilized as a molecular marker for epidemiological studies of vanB2-resistant Enterococcus faecium.