Sequence comparison of Sarcocystis neurona surface antigen from multiple isolates

Sarcocystis neurona is responsible for equine protozoal myeloencephalitis (EPM) and is phylogenetically closely related to Toxoplasma gondii and Neospora spp. There has been major progress in evaluating immunodominant and surface proteins of T. gondii and Neospora spp.; however, there is a paucity of data on the proteins or genes from S. neurona and S. neurona-like parasites. In this study, using reverse transcription-PCR (RT-PCR) and restriction fragment length polymorphism (RFLP) methods, the surface antigen gene 1 (SAG1) from several isolates was characterized by sequence analysis and evaluated for molecular diagnostic identification. AluI restriction digestion of PCR amplicons of the SAG1 gene verified the sequencing results. The putative SAG1 gene from isolates of S. neurona or S. neurona-like parasites varied from 73 to 100% sequence similarity, whereas the SAG1 gene from isolates of Neospora spp. varied from 96 to 98% sequence similarity.