Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Eukaryotic translation initiation factors and regulators.

Nahum Sonenberg1, Thomas E Dever

  • 1Department of Biochemistry and McGill Cancer Centre, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec H3G 1Y6, Canada. nahum.sonenberg@mcgill.ca

Current Opinion in Structural Biology
|February 13, 2003
PubMed
Summary

Structural studies reveal common folds in eukaryotic translation initiation factors, offering insights into protein synthesis mechanisms and regulation across species.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated stress response couples mitochondrial fitness with lineage reprogramming to drive cancer evolution.

Nature cell biology·2026
Same author

Coordinated regulation of mRNA translation and stability by ZC3H7A and ZC3H7B RNA-binding proteins.

Cell reports·2026
Same author

Translational control in the spinal cord regulates gene expression and pain hypersensitivity in the chronic phase of neuropathic pain.

eLife·2026
Same author

4E-BP inhibition ameliorates heart failure through translational upregulation of SERCA2a and modulation of mitochondrial redox signaling in cardiomyocytes.

Redox biology·2026
Same author

The ISR downstream effector ATF4 promotes mGluR-dependent long-term depression and associated behavior.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

4E-BP2-dependent translational control in GABAergic interneurons is required for long-term memory.

Molecular neurobiology·2026

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Genetics

Background:

  • Eukaryotic translation initiation factors (eIFs) are crucial for assembling ribosomes on mRNA.
  • Understanding the structure of eIFs is key to deciphering protein synthesis regulation.
  • Previous research laid the groundwork for detailed structural investigations.

Purpose of the Study:

  • To elucidate the structural basis of eukaryotic translation initiation.
  • To understand the mechanism and regulation of protein synthesis.
  • To explore the evolutionary conservation of the translation machinery.

Main Methods:

  • X-ray crystallography
  • Cryo-electron microscopy (Cryo-EM)
  • Nuclear Magnetic Resonance (NMR) spectroscopy

Related Experiment Videos

Main Results:

  • Identified conserved structural folds among eukaryotic translation initiation factors.
  • Revealed similarities in structural organization across different species.
  • Provided atomic-level insights into ribosome assembly and initiation.

Conclusions:

  • Structural studies of eIFs have significantly advanced our understanding of protein synthesis.
  • Conserved structural elements suggest a common evolutionary origin for translation factors.
  • These findings are vital for comprehending cellular and viral protein synthesis regulation.