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Serglycin proteoglycan expression and synthesis in embryonic stem cells.
Barbara P Schick1, Hon-Chung Keith Ho, Kristin C Brodbeck
1Cardeza Foundation for Hematologic Research, Department of Medicine, Jefferson Medical College of Thomas Jefferson University, 1015 Walnut Street, Philadelphia, PA 19107, USA. barbara.schick@mail.tju.edu
Biochimica Et Biophysica Acta
|February 13, 2003
Summary
Serglycin proteoglycan is found in embryonic stem cells, independent of blood cell development. Its expression and structure change with differentiation, influenced by retinoic acid and cAMP.
Area of Science:
- Biochemistry
- Developmental Biology
- Stem Cell Research
Background:
- Serglycin proteoglycan is typically found in hematopoietic cells.
- Its role in non-hematopoietic cells, particularly embryonic stem cells, is not well understood.
Purpose of the Study:
- To investigate serglycin expression and secretion in undifferentiated murine embryonic stem (ES) cells.
- To determine if ES cell serglycin expression is linked to hematopoiesis.
- To examine the effects of differentiation-inducing agents on ES cell serglycin.
Main Methods:
- Detection of serglycin mRNA and protein in ES cells and embryoid bodies.
- Immunostaining for intracellular localization.
- Analysis of secreted serglycin core protein structure after chondroitinase digestion.
- Treatment of ES cells with retinoic acid (RA) and dibutyryl cAMP (dbcAMP).
Main Results:
- Serglycin mRNA and protein are expressed, synthesized, and secreted by undifferentiated ES cells.
- Serglycin expression was observed in tal-1((-/-)) ES cells, indicating it is not associated with hematopoiesis.
- RA and dbcAMP treatments increased serglycin expression.
- Differentiation treatments altered the structure of the serglycin core protein.
Conclusions:
- Embryonic stem cell serglycin expression is independent of hematopoietic differentiation.
- Serglycin plays a role in ES cell biology and is modulated during differentiation.
- The structure of serglycin core protein is sensitive to differentiation cues.