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[Two restriction fluorescence labeling methods for enhancing the signal-to-noise ratio of cDNA microarray
Rong Shi1, Wen-li Ma, Yan-bin Song
1Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China. shirong@fimmu.edu.cn
Objective:
To study the signal-to-noise ratio (SNR) of two restricted fluorescence labeling methods for examining gene expression profile by microarray hybridization.
Method:
Samples of Saccharomyces cerevisiae mRNA was labeled by traditional reverse transcription method and 2 restriction fluorescent labeling methods using respectively Cy-universal primer and extension incorporated Cy-dNTP. The labeled samples were examined by the microarray, followed by washing and scanning under the same conditions.
Results:
The two restriction labeling methods showed superior results with lowered background and enhanced SNR and sensitivity, and Cy-universal primer labeling presented the best results.
Conclusion:
SNR can be enhanced by the restriction labeling methods, which improve the applicability of microarray technology.