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[In vitro biocompatibility and degradation of nacre/polylactic acid composite artificial bone]
Jin-biao Liu1, Jian-ting Chen, Da-di Jin
1Department of Orthopaedics Surgery, Chengdu General Hospital of Chengdu Command, Chengdu 610083, China.
Objective:
To investigate the in vitro biocompatibility and degradation of nacre/polylactic acid composite artificial bone (NPCB) grafts.
Methods:
Human osteoblast cells were cultured with NPCB discs for observation of the morphological features and cell proliferation by optical microscope and scanning electron microscope. The mass loss of NPCB discs and pH variations of the saline in which the discs were immersed were examined every 2 weeks in a course of 16 weeks in vitro.
Result:
Compared with the negative control group, NPCB showed no visible cytotoxicity and facilitated the growth and proliferation of the osteoblast cells. As the immersion prolonged, gradual decrease in the mass of NPCB was observed accompanied by regular pH alteration of the saline.
Conclusion:
NPCB possesses good in vitro biocompatibility and may be gradually degraded.