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Published on: August 20, 2015
[In vitro culture of human hair follicles and sequential observation]
1Department of Plastic Surgery, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. yenshin@fimmu.com
Objective:
To obtain successful in vitro culture of human hair follicles, and observe their morphological changes in the middle and later period of culture.
Methods:
Human hair follicles were isolated from the scalp resected from patients receiving surgical treatment of the wrinkles, and were cultured in DMEM supplemented with 10% calf serum for up to 30 d. The growth of the follicles, along with their morphological and histological changes, was observed sequentially.
Results:
In vitro culture of human hair follicles was successfully obtained. The follicles showed catagen-like changes in the middle and later period of culture.
Conclusion:
Human hair follicles cultured in vitro tend to transform early into catagen, and this culture method has simplified preparation of routine paraffin section of hair follicles.
