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Updated: Aug 13, 2026

Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
Published on: March 17, 2015
[Expression of tyrosine kinase receptor B in human follicle]
Juan Fu1, Fu-qi Xing, Shi-ling Chen
1Center for Assisted Reproduction, Department of Obstetrics and Gynecology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. fj81609@sina.com.cn
Objective:
To study the expression of tyrosine kinase receptor B(TrkB) in human follicle and explore the significance of the expression.
Methods:
Streptavidin-biotin peroxidase complex immunocytochemistry was employed to determine the expression of TrkB on the oocytes, cumulus granulosa cells (CGCs) or mural granulosa cells (MGCs) obtained from women during controlled ovarian hyperstimulation for in vitro fertilization-embryo transfer (IVF-ET). Western blotting was used to analyze TrkB expression level in CGCs and MGCs.
Results:
The results demonstrated for the first time the presence of TrkB on the surface of human ovary cells including MGCs, CGCs and the oocytes. Higher expression levels of TrkB were detected on the MGCs than on the CGCs.
Conclusion:
It is possible that TrkB tyrosine autophosphorylation in response to neurotrophins can take part in the regulation and modulation of steroidogenesis and oocyte maturation.
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