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Sodium loading changes urinary protein excretion: a proteomic analysis.

Visith Thongboonkerd1, Jon B Klein, William M Pierce

  • 1Core Proteomics Laboratory, Kidney Disease Program, Department of Medicine, University of Louisville, KY 40202, USA. visith.thongboonkerd@louisville.edu

American Journal of Physiology. Renal Physiology
|February 13, 2003
PubMed
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Acute sodium loading alters urinary protein excretion, impacting sodium homeostasis. Researchers identified 45 changed protein components, revealing key changes in renal tubular protein expression during sodium balance regulation.

Area of Science:

  • Nephrology
  • Proteomics
  • Physiology

Background:

  • Plasma sodium concentration is tightly regulated, a process known as sodium homeostasis.
  • Renal tubular protein expression changes may reflect and contribute to sodium homeostasis.
  • Urinary protein analysis offers a non-invasive method to study these renal adaptations.

Purpose of the Study:

  • To investigate changes in urinary protein excretion in response to acute sodium loading using proteomic analysis.
  • To identify specific proteins whose excretion is altered during changes in sodium intake.
  • To understand the role of urinary proteins in maintaining sodium balance.

Main Methods:

  • Rats were subjected to acute sodium loading with hypertonic saline after a control period with deionized water.

Related Experiment Videos

  • Urinary proteins were collected and analyzed using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and Sypro ruby staining.
  • Differentially expressed proteins were identified using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and peptide mass fingerprinting.

    • Main Results:

    • Acute sodium loading significantly increased urinary sodium excretion.
    • Proteomic analysis revealed alterations in the abundance of 45 urinary protein components.
    • Key proteins like neutral endopeptidase, heat shock protein 72, and vacuolar H(+)-ATPase decreased, while albumin and alpha-2u globulin increased.

    Conclusions:

    • Acute sodium loading induces significant changes in urinary protein excretion patterns.
    • These identified proteins are potentially involved in tubular transport and sodium homeostasis.
    • Urinary proteomic profiling provides insights into the physiological adaptations of the kidney to altered sodium levels.