Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ordered ATP hydrolysis in the gamma complex clamp loader AAA+ machine.

Aaron Johnson1, Mike O'Donnell

  • 1Howard Hughes Medical Institute and the Rockefeller University, New York, New York 10021, USA. johnsoa@mail.rockefeller.edu

The Journal of Biological Chemistry
|February 13, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TMEM119+ microglia MHC class I restricted antigen presentation impacts CD8 T cell memory, effector status, and blood-brain barrier disruption during neurotropic virus infection.

Research square·2026
Same author

Virus-specific CD8 T cells rapidly populate and persist in skull bone marrow after brain infection.

Research square·2026
Same author

A randomized double-blind placebo-controlled phase I/II clinical trial of a human papillomavirus therapeutic vaccine, PepCan, for reducing head and neck squamous cell carcinoma recurrence.

Oncotarget·2026
Same author

A Randomized Double-Blind Placebo-Controlled Phase I/II Clinical Trial of a Human Papillomavirus Therapeutic Vaccine, PepCan, for Reducing Head and Neck Cancer Recurrence.

medRxiv : the preprint server for health sciences·2026
Same author

Directional Perilaryngeal Functional Muscle Network Indicates Sequential Patterns of Muscle Coordination During Vocal Tasks.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same author

Infection and chronic disease activate a systemic brain-muscle signaling axis.

Science immunology·2024

The gamma complex, crucial for DNA replication, uses its delta' stator to hydrolyze ATP. This process occurs in a specific order, essential for loading beta sliding clamps.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • The gamma complex is essential for DNA replication, facilitating the loading of beta sliding clamps onto DNA.
  • It comprises a heteropentameric structure with distinct subunits: gamma motor subunits, a delta wrench, and a delta' stator.

Purpose of the Study:

  • To elucidate the role of the delta' stator in the ATPase activity of the gamma complex.
  • To understand the mechanism and order of ATP hydrolysis within the gamma complex during clamp loading.

Main Methods:

  • Structural analysis of the gamma complex.
  • Site-directed mutagenesis of key arginine residues in the gamma complex.
  • Biochemical assays to measure ATPase activity.

Related Experiment Videos

Main Results:

  • The delta' stator contains a catalytic arginine that facilitates ATP hydrolysis on the adjacent gamma(1) subunit.
  • Mutation of arginine 169 in gamma abolished ATPase activity, indicating a sequential hydrolysis order.
  • ATP hydrolysis occurs in reverse order of ATP binding, with ATP site 1 hydrolysis dependent on sites 2 and/or 3.

Conclusions:

  • The delta' stator is integral to the motor function of the gamma complex, contributing catalytically to ATP hydrolysis.
  • The sequential and ordered ATP hydrolysis is critical for the efficient function of the clamp loader complex.
  • Findings provide insights into the mechanism of other related clamp loader systems.