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The development of a STS marker linked to a yellow rust resistance derived from the wheat cultivar Moro
H. Smith1, D. Koebner, A. Boyd
1Cereals Research Department, John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK.
Abstract:
A sequence-tagged-site (STS) marker has been developed for a gene conferring yellow rust resistance originating from the wheat cultivar Moro. The single, dominant, seedling yellow rust resistance gene, designated YrMoro, was mapped to the group 1 chromosomes. The STS marker was developed from an AFLP band which cosegregated with the YrMoro gene. Sequence-specific primers were made which incorporated the selective bases of the AFLP primers, plus 16 and 17 additional bases extending into the AFLP band. This simple, PCR-based marker will allow wheat breeders to pyramid this resistance gene, along with other resistance genes, into a single wheat genotype.
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