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Megagametophyte-derived linkage maps of white spruce ( Picea glauca) based on RAPD, SCAR and ESTP markers
I. Gosselin1, Y. Zhou, J. Bousquet
1Centre de recherche en biologie forestière and Chaire de recherche du Canada en génomique forestière et environnementale, Pavillon Charles-Eugène-Marchand, Université Laval, Ste-Foy Québec, Canada G1K 7P4.
Abstract:
We have constructed linkage maps for two parents of white spruce [ Picea glauca (Moench) Voss]. Haploid megagametophytes from 92 and 96 seeds of parents M2 and 80132, respectively, were analysed with RAPD, SCAR and ESTP markers. Fragments segregating in a 1:1 Mendelian ratio were classified and mapped using MAPMAKER, GMENDEL and JOINMAP. For M2, the analysis with JOINMAP resulted in 165 loci (152 RAPDs, 3 SCARs and 10 ESTPs) mapping to 23 linkage groups and covering 2,059.4 cM(Kosambi function, K). For 80132, the analysis resulted in 144 loci (137 RAPDs, 1 SCAR and 7 ESTPs) mapping to 19 linkage groups and covering 2,007.7 cM(K). The maps covered 87 and 73% of the entire genome of parents M2 and 80132, respectively. Similar results were obtained with MAPMAKER and GMENDEL. A comparison was made between the two individual maps and 16 loci were shared between the two maps.