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Related Experiment Videos

Transpecific microsatellites for hard pines.

M. Shepherd1, M. Cross, L. Maguire

  • 1Cooperative Research Centre for Sustainable Production Forestry, Centre for Plant Conservation Genetics, Southern Cross University, P.O. Box 157 Lismore, NSW 2480, Australia, mshepher@scu.edu.au

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|February 13, 2003
PubMed
Summary

Cross-species microsatellite markers are valuable for large plant genomes. This study found 58% of tested microsatellites amplified in slash pine and Caribbean pine, with 23 showing useful genetic variation.

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Area of Science:

  • Genetics
  • Plant Science
  • Bioinformatics

Background:

  • Microsatellites are valuable genetic markers but difficult to develop in large plant genomes.
  • Cross-specific utilization of microsatellites offers an efficient alternative for marker discovery.

Purpose of the Study:

  • To assess the cross-specific amplification and polymorphism of microsatellite markers in two New World hard pine species.
  • To evaluate the utility of these markers for phylogenetic analysis within the Pinus genus.

Main Methods:

  • Fifty microsatellite markers were tested for amplification and polymorphism in Pinus elliottii var. elliottii and Pinus caribaea var. hondurensis.
  • Allele length variation and repeat motif changes were analyzed.
  • Comparison with soft pine (subgenus Strobus) markers was performed.

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Main Results:

  • Twenty-nine (58%) microsatellite markers successfully amplified in both hard pine species.
  • Twenty-three of the amplified markers (79%) were polymorphic, indicating genetic variation.
  • Soft pine markers amplified but were not polymorphic in the hard pines.
  • Mutational changes provided insights into Pinus spp. phylogenetic relationships.

Conclusions:

  • A significant proportion of microsatellites developed for other Pinus species are transferable and polymorphic in slash pine and Caribbean pine.
  • These cross-specific microsatellites are useful for population genetics and phylogenetic studies in these hard pines.
  • No ascertainment bias was detected in the marker set.