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A simple method for nicardipine hydrochloride quantification in plasma using solid-phase extraction and

Catarina Marques Fernandes1, Francisco José Baptista Veiga

  • 1Laboratory of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal.

Biomedical Chromatography : BMC
|February 13, 2003
PubMed
Summary

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A new reversed-phase liquid chromatography method accurately measures nicardipine hydrochloride (NC) in rabbit plasma. This analysis revealed rapid absorption and a short half-life for NC in rabbits.

Area of Science:

  • Analytical Chemistry
  • Pharmacokinetics
  • Biomedical Science

Background:

  • Nicardipine hydrochloride (NC) is a calcium channel blocker used to treat hypertension.
  • Accurate quantification of NC in biological matrices is crucial for pharmacokinetic studies.

Purpose of the Study:

  • To develop and validate a simple, sensitive, and rapid reversed-phase liquid chromatography (RPLC) method for determining nicardipine hydrochloride in rabbit plasma.
  • To investigate the pharmacokinetic profile of NC following oral administration in rabbits.

Main Methods:

  • Plasma samples were prepared using solid-phase extraction (SPE) with C18 cartridges.
  • Separation was achieved on a C18 column with a mobile phase consisting of acetonitrile, sodium phosphate buffer, and methanol.
  • Quantification was performed using ultraviolet (UV) detection at 254 nm.

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Main Results:

  • The method demonstrated linearity over the range of 5-100 ng/mL (R² = 0.9993).
  • Extraction efficiency ranged from 77.56% to 84.23%, with good intra- and inter-day precision (RSD < 9.38%).
  • Accuracy was confirmed by bias percentages within +/- 5.2%.
  • In vivo study showed rapid absorption and a short half-life (t½ = 1.34 h) for NC in rabbits.

Conclusions:

  • The developed RPLC method is suitable for the reliable determination of nicardipine hydrochloride in rabbit plasma.
  • Nicardipine hydrochloride is rapidly absorbed and exhibits a short duration of action in rabbits.