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Related Concept Videos

Anatomy of the Ear01:16

Anatomy of the Ear

Auditory sensation, commonly called hearing, involves the transformation of sonic waves into neural impulses facilitated by the structures of the auditory organ. The prominent, flesh-like structure on the side of the head, called the auricle, directs sound waves towards the auditory canal. The auricle is often mislabeled as the pinna, a term more aligned with mobile structures like a feline's external ear. The auditory canal penetrates the cranium via the external auditory meatus of the...

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Related Experiment Video

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The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration
12:21

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Published on: November 26, 2015

A novel technique for inducing local inner ear damage.

Takayuki Nakagawa1, Tae Soo Kim, Norihiko Murai

  • 1Department of Otolaryngology-Head and Neck Surgery, Kyoto University, Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, 606-8507 Kyoto, Japan. tnakagawa@ent.kuhp.kyoto-u.ac.jp

Hearing Research
|February 14, 2003
PubMed
Summary

Researchers developed a new method to induce hair cell apoptosis in mouse inner ear models using neomycin. This technique facilitates genetic studies into hair cell degeneration mechanisms.

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Area of Science:

  • Ototolaryngology
  • Neuroscience
  • Genetics

Background:

  • Mice are crucial for inner ear research due to genomic advancements.
  • Challenges exist in studying mouse inner ear hair cell degeneration, including resistance to ototoxic agents and surgical difficulties.

Purpose of the Study:

  • To establish a reliable mouse model for inducing apoptotic cell death in inner ear sensory epithelia.
  • To investigate the mechanisms of hair cell apoptosis using genetic tools.

Main Methods:

  • Induction of apoptosis via neomycin injection into the posterior semicircular canal.
  • Assessment of apoptotic cell death using Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay.
  • Evaluation of caspase dependency using caspase-inhibitors.

Main Results:

  • Local neomycin application successfully induced apoptotic cell death in auditory and vestibular epithelia.
  • TUNEL assay confirmed apoptosis with a defined time course.
  • Caspase-inhibitor significantly reduced TUNEL-positive cells, confirming caspase-dependency.
  • The surgical approach was straightforward, and sham operations showed no significant apoptosis.

Conclusions:

  • A novel, effective, and surgically accessible mouse model for inducing inner ear hair cell apoptosis has been developed.
  • This model supports the use of genetic tools to explore the mechanisms underlying hair cell apoptosis.
  • The findings highlight the caspase-dependent nature of neomycin-induced hair cell death.