A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation
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1Section of Molecular Cell and Developmental Biology (A6700), Institute for Cellular and Molecular Biology, University of Texas at Austin, Bio 311, Austin, TX 78712, USA.
Abstract:
Cosmid vectors containing a dominate selectable marker (ble) for complementation cloning of genes in Chlamydomonas reinhardtii were created. The usefulness of these vectors, which differ in the orientation of the ble cassette, was demonstrated by transforming C. reinhardtii to phleomycin resistance, by constructing a large library (approximately 5 x 10(5) recombinants) in one of them using DNA from a C. reinhardtii mutant, and by transforming C. reinhardtii with recombinant cosmid clones and pools.
