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Two-photon molecular excitation imaging of Ca2+ transients in Langendorff-perfused mouse hearts
Michael Rubart1, Exing Wang, Kenneth W Dunn
1Wells Center for Pediatric Research, Indianapolis, Indiana 46202, USA. mrubartv@iupui.edu
American Journal of Physiology. Cell Physiology
|February 14, 2003
Abstract:
The ability to image calcium signals at subcellular levels within the intact depolarizing heart could provide valuable information toward a more integrated understanding of cardiac function. Accordingly, a system combining two-photon excitation with laser-scanning microscopy was developed to monitor electrically evoked [Ca(2+)](i) transients in individual cardiomyocytes within noncontracting Langendorff-perfused mouse hearts. [Ca(2+)](i) transients were recorded at depths