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Inhibition of gammaherpesvirus replication by RNA interference
1Department of Molecular and Medical Pharmacology, University of California at Los Angeles, Los Angeles, California 90095, USA.
Journal of Virology
|February 14, 2003
Abstract:
RNA interference (RNAi) is a conserved mechanism in which double-stranded, small interfering RNAs (siRNAs) trigger a sequence-specific gene-silencing process. Here we describe the inhibition of murine herpesvirus 68 replication by siRNAs targeted to sequences encoding Rta, an immediate-early protein known as an initiator of the lytic viral gene expression program, and open reading frame 45 (ORF 45), a conserved viral protein. Our results suggest that RNAi can block gammaherpesvirus replication and ORF 45 is required for efficient viral production.