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Decreased syndecan-2 expression correlates with trichostatin-A induced-morphological changes and reduced tumorigenic

Yeonhee Kim1, Haein Park, Yangmi Lim

  • 1Department of Life Sciences, Division of Molecular Life Sciences and Center for Cell Signalling Research, Ewha Womans University, Seoul, Korea.

Oncogene
|February 14, 2003
PubMed

Insights

Histone deacetylase inhibitor trichostatin A (TSA) alters colon carcinoma cell morphology. Reduced syndecan-2 expression correlates with these changes and decreased tumor cell growth.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Histone deacetylase (HDAC) inhibition is known to induce morphological alterations in transformed cells.
  • Colon carcinoma cell lines exhibit characteristic growth patterns that can be modulated by external factors.

Purpose of the Study:

  • To investigate the specific effects of trichostatin A (TSA), a histone deacetylase inhibitor, on the morphology of colon carcinoma cell lines.
  • To explore the correlation between TSA-induced morphological changes and the expression of syndecan-2.

Main Methods:

  • Treatment of human colorectal carcinoma cell lines (KM1214 and KM12SM) with trichostatin A (TSA).
  • Assessment of cell morphology, actin filament organization, and expression/localization of E-cadherin and beta-catenin.
  • Analysis of syndecan-2 expression levels and its role via antisense cDNA downregulation.
  • Evaluation of anchorage-independent growth.

Main Results:

  • TSA treatment induced distinct morphological changes in colon carcinoma cells, leading to flattened monolayers with clear cell boundaries and increased actin organization.
  • Syndecan-2 expression was dramatically reduced following TSA treatment, correlating with the observed morphological shifts.
  • Downregulation of syndecan-2 expression mimicked TSA-induced morphological changes and reduced anchorage-independent growth.

Conclusions:

  • Reduced syndecan-2 expression is closely associated with TSA-induced morphological alterations in colon carcinoma cells.
  • The modulation of syndecan-2 expression by HDAC inhibition may contribute to reduced tumorigenic activity in colon cancer.

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