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[Experimental study on inhibitory effect of rutin against platelet activation induced by platelet activating factor
Wen-mei Chen1, Ming Jin, Wei Wu
1Beijing Institute of Heart, Lung and Vascular Diseases, Beijing 100029.
Objective:
To explore the inhibitory effect and mechanism of rutin against platelet activating factor (PAF) induced platelet aggregation, 5-HT release and intra-platelet free calcium concentration.
Methods:
The rate of washed rabbit platelet (WRP) aggregation was measured by turbidimetry and O-phthaldialdehyde (OPT) fluoro-spectrophotometry (FSPM) was used to determine 5-HT content. The intraplatelet free calcium concentration was measured with Fura-2/AM FSPM assay.
Results:
Rutin in vitro was concentration-dependently inhibiting PAF (9.55 x 10(-9) mol/L) induced WRP aggregation, the IC50 of 5-HT release was 0.73, 1.13 mmol/L respectively and the intraplatelet free calcium concentration elevation evoked by PAF (4.78 x 10(-10) mol/L) were inhibited by 68.3, 136, 274, 545 mumol/L of rutin dose-dependently.
Conclusion:
Rutin could inhibit PAF induced platelet aggregation, 5-HT release and the increase of intraplatelet free calcium.
