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[Study on the structure and activity of new phenolic acid compounds from Erigeron breviscapus]
1Department of Phytochemistry, College of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Aim:
To isolate and identify the active constituents of Erigeron brevisapus (Vant.) Hand.-Mazz.
Methods:
The chemical constituents were isolated by silica gel column chromatography and two new compounds were obtained. Their structures were elucidated by IR, MS, 1HNMR, 13CNMR, DEPT and 2DNMR. The injury of BCMEC (bovine cerebral microvascular endothelial cell) was determined by lactic dehydrogenase (LDH), the ability of the drugs anti-oxidation and scavenging oxidation of free radical was measured by colorimetric method.
Results:
Two new compounds have been identified as 1-O-methyl-3, 5-O-dicaffeoyl quinic acid methyl ester (III) and 5-O-caffeoyl quinic acid butyl ester (IV).
Conclusion:
Compounds III and IV are new compounds. Compound III can protect BCMEC injury by LPC.